원문정보
초록
영어
Recently, as the requirement for a highly purified nucleic acid has been increased in various fields including biotechnology, diagnostics, pharmacology and metabolomics, attempts have been made to isolate a nucleic acid more conveniently and rapidly from a variety of biological samples. Here we described evaluation of an automatic nucleic acid isolation system from diverse sample types using ExiPrepTM 16 Plus/ Pro(Bioneer Corp., Korea). This is based on the silica coated magnetic particle technique combined with chaotropic reagent which can binds to targetmolecules for example, DNA or RNA. ExiPrepTM 16 Plus/ Pro could extract the DNA or RNA from 16 samples at the same time from whole blood, tissue, cultured cells and any clinical samples within 30-60 min. The quantity and purity of the isolated nucleic acid was calculated with Nano-Drop spectrophotometer(Thermo Scientific) and the quality was evaluated with gel electrophoresis and real-time PCR. ExiPrepTM 16 Plus/ Pro provides user convenience, easy operation and reproducible result for the molecular biological research area.