earticle

영어

Citrus fruits contain 35~55% juice and rest as solid biomass, only 5% of this biomass utilizes as silage and rest are treated as waste material. The management of the waste is very expensive and non productive. Thus we have focused to produce GABA and antimicrobials by fermentation using lactic acid bacteria. The present aim is to develop an effective and simple process to convert the citrus waste into value added commodity. The citrus silage of one to three years maturity level was collected and evaluated the bio-organic properties. For screening of production of antimicrobials, we have used seven strains from KCCM and 37 strains isolated from porcine feces. The culture supernatant was used to evaluate the antimicrobial activity against E. coli O-157, S. aureus, E. coli K88, and Salmonella spp. S. gallinarum, S. enteritidis, and S. typhimurium. Out of all screened bacteria 23 strains were found to be antagonistic for S. enteritidis and S. typhimurium. The three strains namely L. reuteri LA17, B. subtilis 3-3, L. brevis IFO 12005 were secreted antimicrobials for S. enteritidis and S. typhimurium. In addition to that Factorial Design was used to optimize the GABA production from same silage, at different nitrogen source.