원문정보
초록
영어
In the present study, effect of 7′-phloroeckol isolated from Ecklonia cava on the growth of human epidermal keratinocyte growth was investigated by measuring cell proliferative and anti-apoptotic activities in HaCaT cells. The cell proliferative and anti-apoptotic activities were evaluated using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay and Western blotting. Treatment with the 7′- phloroeckol increased the proliferation of HaCaT cells by 259.2% at a concentration of 0.5 μM compare with control. The protein expression levels of phosphorylated-extracellular signal-regulated kinases (p-Erk) and p-Akt were significant increased by 7′-phloroeckol. Apoptosis related Bcl-2/Bax ratio was also increased in a dose dependent manner. Consequently, 7′-phloroeckol has the potential to promote the human epidermal keratinocyte through stimulating proliferation and preventing apoptosis.