earticle

영어

Sep-Pak C18 cartridges were investigated to evaluate their ability to purify anti-photoaging compound extracted from Ficus deltoidea (FD) leave. This cartridge is enable efficient purification of peptides and small proteins before analytical techniques. Several studies have been reported that Ficus species contain polypeptide compounds that known to have strong antioxidant properties. In our previous data FD water extract shows high anti-photoaging and anti-melanogenic activity. From this observation it might be a useful idea to purify the peptide compound from FD extract that responsible to protect UVB-induced skin cells from photoaging. Therefore, in the present study, we investigate the effect of cleanup FD extract on the expression of TNF-α and MMP-1, and collagen synthesis in UVB irradiated cultured human fibroblast and keratinocytes. FD extract were diluted with 1% trifluoroacetic acid (TFA), 0.1 M Amm. Bicarb (ABC) and distilled water separately, followed by filtration and dried using freeze dryer, prior to the Sep-Pak C18 cleanup. These buffers represent the different properties of peptide eluted from the cartridges. The treatment of all samples inhibited UVB-induced TNF-α expression. Distilled water is the preferred diluents that reduce TNF-α expression induced by UVB irradiation more than 70% at 0.005% compare to dexamethason. The use of sample diluents such as distilled water and ABC were reduced UVB-induced MMP-1 expression in a dose-dependent manner. The treatment of fibroblast with both samples were also increased the reduction of UVB-induced collagen synthesis. Therefore, these results point to the potential use of Sep-Pak C18 to cleanup FD extract for purifying the anti-photoaging compound.