earticle

영어

We tried to analyze effects of Red Ginseng Oil (RGO) and Fractionated RGO (RGOf) on skin barrier function for both in vitro and in vivo. RGO was prepared by using supercritical extraction of the cake produced after extraction of red ginseng with boiling water. RGOf was prepared by hexane fractionation. The effect of RGO on the recovery of tape stripped skin barrier was measured by TEWL by using hairless mouse. Treatment of 0.1% RGO and RGOf showed the fastest recovery of skin barrier function expressed by TEWL compared to those of vehicle and 1% RGO. Alleviation effect of RGO on inflammation of human skin was also evaluated. RGOf significantly alleviated skin inflammation induced by UVB irradiation. In addition, the pro-inflammatory cytokines TNF- α was inhibited more than 60% in HaCaT keratinocytes by RGOf. And RGO and RGOf stimulated gene of differentiation markers, such as TG-1, Keratin 10 and involucrin. Collectively data shown in this study suggest that RGOf have strong skin protective activities by stimulating the epidermal homeostasis and preventing UVB induced skin inflammation.