earticle

영어

It has been reported that singe-strand (ss) DNA breaks could be photosensitized by some drugs. However, conventional methods to monitor the genotoxicity of the photosensitizing drugs are limited to the application for a high throughput screening of the photo-toxic drug because they are time consuming for the biological or chemical modification of the tested DNA and instrument-oriented. Therefore, We have investigated a colorimetric method to visualize ss DNA breaks using gold nanoparticles (AuNPs). ss DNA is known to physically adsorb onto AuNPs and prevent them from salt-induced aggregation. However, AuNPs mixed with UV irradiated ss DNA in the presence of photosensitizing drugs were aggregated after the salt addition into the mixture, resulting in red-to-purple color change. The extent of AuNP-aggregation was dependent on the UV-irradiation time and concentration of photosensitizers. In current, we are investigating the principle for the aggregation of AuNPs by only the ss DNA which were irradiated by UV light in the presence of ss DNA break photosensitizing drugs. The detail experimental results and working principle will be presented.