earticle

영어

Over the last decades, significant efforts have been made to develop detection tools for monitoring hormones because of their biomedical importance. Well established detection methods for hormones are normally based on immunomeric assays. They, however, involve the use of labor-intensive and time consuming protocols. Here, we demonstrated a method to monitor the activity of human parathyroid hormone receptor (hPTHR), which belonging to B class G-protein coupled receptor (GPCR) family, using polypyrrole nanoparticle (PPyNP)-field effect transistor (FET) which allowed the development of a high-performance hormone sensor. In this work, PPyNP-FETs with uniform particle diameters of 20, 50 and 100 nm were functionalized with hPTHR produced from E. coli and detect target hormone peptide hormone, hPTH, with high sensitivity as low as picomolar concentration. In addition, hPTHR-PPyNP-FET showed high selectivity for target hormone detection and successfully recognized deletion form of hPTHs. This work may allow high-performance hormone sensor that can be used for the practical application including disease diagnosis and drug discovery. This method should also provide a powerful tool for basic research on GPCRs.