earticle

영어

Electric Cell-substrate Impedance Sensing (ECIS) has been currently emerging as a novel, non-invasively and sensitive techniques in detection and analyzation of cellular responses to chemical and biological agent. In this study, we provide the fabrication and measurement techniques of ECIS system to detect cell viability and cell-toxicity screening. A bi-layer of Au/Ti was first deposisted and etched to form the sensing area, lead trace and the contact pad. The sensing microelectrodes were then photolithographically defined by a thin dielectric layer of SU-8 photoresist. For carrying out the detection and measurement, the HeLa cells were cultured on these electrodes and alternative current (AC) with the frequency from 100Hz to 100KHz was applied. The cellular spreading, morphology and multiplication was monitored by the impedance changes of the obtained electrical signal. At last, the anti-cancer drug was applied to watch for the cell-toxicity screening. With this design of ECIS system, we are able to use the commonly used ECIS-based technique to integrate into the microfluidic devices for many various purposes. Acknowledgment: This subject is supported by the Korea Science and Engineering Foundation (KOSEF) grant funded by the Korea government (MEST) (No. 20100020657)