earticle

영어

The abnormal aggregation of β-amyloid peptide (Aβ) in the brain is a major histopathological feature of Alzheimer’ disease (AD). Herein, we report on microfluidic dissociation and clearance analysis of preformed Aβ aggregates for parallel screening of aggregate destabilizers in a high-throughput manner. As a proof of the concept for the microfluidic platform, we investigated (1) microfluidics-based clearance of metal ion-induced Aβ aggregates using different types of metal chelators, (2) the clearance effect of deferoxamine on Aβ aggregates within microchannels, (3) comparison between destabilized Aβ dissociated from preformed A β aggregates and remaining deposits within the microchannels both before and after the clearance, and (4) secondary structure change in Aβ deposits by the clearance treatment. The microfluidics-based clearance system should be suitable for high-throughput screening of drug candidates to enhance the clearance of Aβdeposits for AD therapy prior to their in vivo evaluation.