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포스터 발표 : 바이오센서 및 바이오칩

Human bitter taste receptor functionalized-CPNT-FET as an artificial taste sensor mimicking human taste system

원문정보

Hyun Seok SONG, Sang Hun LEE, Oh Seok KWON, Eun Hae OH, Sae Ryun AHN, Jyongsik JANG, Tai Hyun PARK

한국생물공학회 한국생물공학회 학술대회 2011년도 한국생물공학회 춘계학술발표대회 2011.04 pp.260-260
피인용수 : 0건 (자료제공 : 네이버학술정보)

초록

영어

Artificial taste sensors, so called ‘lectronic tongues’ have been widely used in food and beverage industry. The electronic tongues usually utilize synthetic materials for the recognition of analytes. In this reason, they cannot mimic the biological features of the human taste system. To overcome these limitations, we applied biological human taste receptor for the development of artificial taste sensor. Here, we demonstrated human bitter taste receptor, hTAS2R38, functionalized conducting polymer nanotube(CPNT)-field effect transistor (FET) mimicking human taste system. Each taster (PAV) and non-taster (AVI) haprotype of hTAS2R38 genes were cloned into bacterial expression vector and then expressed in E. coli at high-level. The receptors were then immobilized on CPNT-FET sensor platform. The PAV-CPNT-FET responded to target bitterness compounds, phenylthiocarbamide (PTC) and propylthiouracil (PROP), with high sensitivity down to concentration as low as 10 fM. However, AVI-CPNT-FET did not stimulated by target bitter tastants. This artificial taste sensor showed very similar performance with human taste system and may offer a powerful tool for basic research on taste receptors.

키워드

저자정보

  • Hyun Seok SONG School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Sang Hun LEE School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Oh Seok KWON School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Eun Hae OH School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Sae Ryun AHN School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Jyongsik JANG School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.
  • Tai Hyun PARK School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742 Korea.

참고문헌

자료제공 : 네이버학술정보

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