earticle

영어

The human immunodeficiency virus (HIV) pandemic mainly affects developing countries, where the Joint United Nations Programme on HIV/AIDS (UNAIDS) estimates suggest that less than 1 in 10 people are aware of their HIV sero-status. In order to enhance epidemiological surveys, prevention programs, and therapeutic interventions, development of specific, rapid, and convenient diagnostic detection systems for HIV is still warranted. Here we report the direct detection of HIV particles using broadly HIV-1 neutralizing gp120 antibody (2G12, gp120MAbs)-conjugated magnetic beads (MBs) and fluorescent nanosized polymeric beads (FNBs). As a model target, noninfectious pseudo HIV particles (pHIVPs) were produced through cotranspected HEK293 by the two plasmids, pCMV-dR.8.74 and pDOLHIVenv, which contain Gag and Pol, and Env of HIV-1, respectively. In the presence of pHIVPs, addition of gp120MAbs-FNBs and gp120MAbs-MBs leads to the formation of sandwich complex, which can be easily isolated and concentrated by common magnet separation. We demonstrate the ability of detecting HIV-1 particles specifically and directly with low sample volume (less than 100 μL) and rapidity (less than 1.5 h). These results suggest that broadly HIV-1 neutralizing antibodies coupled to nanobeads can be employed for the direct detection of HIV-1 particles with potential implication for the development of specific, rapid, and convenient diagnostic systems.