원문정보
초록
영어
Stable contacts between biomolecules and nano-materials are strongly required to design and characterize the bio-nano interface for many applications, such as medical implants, drug delivery, and biosensors. Although there are various strategies to construct the bio-nano interface to fabricate advanced devices with long lifetime, most of strategies are difficult to control the biomolecular binding and maintain the biological activity onto surface of nano-materials. Herein, we demonstrated the powerful and effective approach to attach organophosphrous hydrolase (OPH) onto graphene-based nanohybrids (RGO-Au) through site-specific interaction. The gold binding peptide (GBP)-OPH/RGO-Au was characterized by scanning electron microscopy and circular dichroism (CD). The secondary structure of enzymes after attachment onto RGO-Au represented negligible changes with the GBP. In addition, the GBP-OPH/RGO-Au hybrid were applied into biosensors for detecting organophosphorous compounds.