원문정보
초록
영어
In this study, to identify proteins involved in tumor progression and to deeply understand mechanism underlying melanoma metastasis, comparative proteome analysis on two melanoma cell strains with low and high metastatic potentials, WM793 and 1205LU, respectively, was performed using a combined three-dimensional difference in gel electrophoresis (3-D DIGE) technology [1,2]. The majority of protein level changes are closely associated with many aspects of the pathophysiology of cancers, such as cell death and growth, or tumorigenesis. In addition, cellular pathways related in oncogenes (JNK, c-myc, and N-myc) and tumor suppressor genes (p53 and TGF-β) were found to be prevalent metastatic melanoma progression by the Ingenuity Pathway Analysis. These networks showed substantial overlap and connectivity with the main biological theme of cell death and cancer. Thus the use of more comprehensive proteins profiling to analyze genetically very similar melanoma cell lines can contribute to the identification of novel metastatic biomarkers and provide new insights into the protein pathways involved in melanoma metastasis. [This work was supported by the Basic Science Research Program (2010-0008826) and Converging Research Center Program (2009-0093652) through the National Research Foundation of Korea (NRF) funded by the Ministry of Education, Science and Technology].