earticle

영어

Animal cells are sensitive to both DO and shear stress. First of all, we investigated O2 transfer rate and shear stress of bubble column bioreactors. In the reactors, kLa increased with aeration rate and decreased with the size of bubbles. As far as the bubble size is concerned, small bubbles showed higher O2 transfer than large bubbles. Small bubbles, however, caused shear stress problem that damaged CHO cells. Similar phenomenon was observed in shaking flasks. In well plates or T-flasks, O2 transfer rate was higher in twist-shaking than in tilted-shaking. However, cell growth and viability of twisted flasks were lower than that of tilted ones. At the same time, it was observed that cells were aggregated more in twisting than in tilting. In order to investigate the shear stress marker of CHO cells, 2-D analysis was performed. HSP90-β and G protein could be included in several proteins that might be used as anchorage or shear index proteins. Relationship between oxygen transfer efficiency and shear stress will be presented in this paper.