earticle

영어

Primary human hepatocytes are clinically used for transplantation or in bioartificial liver devices for the treatment of patients with liver failure. Bioartificial liver support systems (BALSS), which employ freshly isolated primary hepatocytes, presently severe logistic difficulties in the continuous supply of primary hepatocytes. Cryopreserved primary hepatocytes that are thawed as required would answer this problem.1)-3) Investigation was carried out with different concentrations of dimethyl sulfoxide (DMSO) with hormonally-defined medium (HDM). Various solutions of histidine-tryptophan-ketoglutarate (HTK), fetal bovine serum (FBS) and sucrose, were used as cryopreservation solutions. In this study, initial dispersed hepatocytes. After thawing viability, plating efficiency, urea synthesis and albumin secretion were measured to assess the effects of freezing methods. The result shows that cryopreserved thawed hepatocytes with HTK solutions enhance hepatocytes cell viability and cell attachement,hence good for hepatocyte cryopreservation. HTK solution in presence of 200mM sucrose showed significantly increase in urea secreation, on the other hand substitution of BME amino acids and BME vitamins does not show any significant.