earticle

영어

Cyclo (His-Pro) (CHP) is a naturally occurring cyclic dipeptide structurally related to thyrotropin-releasing hormone (TRH). CHP was efficiently obtained from soybean meal by hydrolysis using flavourzyme and alcalase. In this study, anti-diabetic effects of CHP were investigated in a Type II diabetic animal model (C57BL/6J ob/ob). When the ob/ob mice were administrated with CHP (2 mg/kg BW, 24 days), blood glucose levels decreased to approximately 57% that of the control mice. In the oral glucose tolerance test, blood glucose levels were restored to its baseline after 120 min by CHP treatment, although blood glucose levels increased significantly after 30 min. Total plasma cholesterol and triglyceride concentrations in ob/ob mice treated with CHP were significantly decreased (2-fold), compared with those in control ob/ob mice. In contrast, insulin levels in plasma were significantly increased (2-fold) in CHP-treated ob/ob mice. Moreover, a histopathological examination showed that CHP administration could restore the impaired β -cells of pancreas to the normal level. In addition, pancreatic β-cells (in CHP-treated and stopped groups) were strikingly restored on 72nd and 130th day, respectively. From these results, it is demonstrated that CHP could be effective for regulating blood glucose to normal level by increasing plasma insulin level in ob/ob mice. Conclusively, we suggest that this compound could be a candidate material as a dietary supplement to control hyperglycemia in patients suffering from type II diabetes.