원문정보
초록
영어
TypeⅠ diabetes is caused by progressive autoimmune destruction of insulin-producing cells (IPCs). Various kinds of stem cells can be used for the preparation IPCs. However, application of stem cells for cell therapy is usually dependent upon donor ages. Accordingly, the growth and differentiation potential of periosteum-derived progenitor cells (PDPCs) obtained from aged fifty, sixty, and seventy into IPCs was investigated in this study. PDPCs have been known to be another kind of mesenchymal stem cells (MSCs) and are expected to be used as a cell source to make IPCs. Our results demonstrated that the cell number, viability and morphology of PCPCs were not age-dependent. Immunofluorescence analysis of b-cell marker (insulin) demonstrated no significant differences in the differentiation by donor age. Insulin was detectable at mRNA levels after induction. Moreover, induced PDPCs could release insulin in response to glucose in vitro. In conclusion, it was found that the differentiation capability of PDPCs into IPCs was not affected by donor ages.