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영어

An angiogenic therapy based on adipose-derived stem cells (ASCs) in an ischemic model has been reported. we have established a 3-D cell mass (3DCM) culture to differentiate human ASCs into vascular cells controlling cell adhesion. We found from a human angiogenesis protein array that the 3DCM composed of hASCs induced hypoxic conditions and expressed angiogenic factors, such as VEGF and angiogenin. VEGF was up-regulated in 3DCM in the presence or absence of fetal bovine serum. as compared with monolayered hASCs cultured on TCP. The extracellular matrix plays an important role in modulating vessel formation by changing its composition and structure to induce endothelial migration. We make a plan to identify the secretome of 3DCMs culture through proteome analyses and apply for a angiogenesis therapy following examination in mouse ischemia model.