원문정보
초록
영어
Adipose tissue-derived stem cells (AT-MSCs) can be extracted from adipose tissue and obtained by a less invasive method and in larger quantities compared with bone marrow–erived MSCs. And many studies have demonstrated that AT-MSCs have differentiate potential to neuronal cells by treating mechanical and chemical methods. The aim of this study is to investigate the effects of sonic vibration (SV) on AT-MSCs and to explore their neuronal differentiation potential. We decided AT-MSCs were exposed to sonic vibration during 4 days at 40 Hz, 1.0 V. After stimulation, we found the differences between sonic vibration exposed group and on-exposed group in terms of cell morphology and RT-PCR electrophoresis products. In the sonic vibration exposed group, RT-PCR indicated that expression level of neuronal markers including NeuroD1, Map2 and NF-L is significantly higher than non-exposed group. So these results suggest that sonic vibration affects MSCs to induce the neuron-like stem cells. And adipose tissue may be an alternative source of stem cell therapy for nervous system injury.