원문정보
초록
영어
Various medical devices have been researched and have been developed for drug transfer to body. At this time, they have been in vitro evaluated with cell lines (L929, MRC5, WI38, Vero, C-13) for predicts of their biocompatibility in vivo. However, the results of experiments in mesenchymal or epithelial cell culture could not be similarly considered in vivo immune reaction. In this study, we evaluated elutions in cell lines (L929, Jurkat, U937) culture and then the result was compared with in vivo results of injection in guinea pig skin. The elution could not induce erythema, but only positive control induce erythema at guinea pig skin test. But three types of cells showed the differences in the secretion of cytokines (TNF-α, INF-γ) and in survival rate for the HDPE elution, respectively. The results of Jurkat (lymphocyte) and U937 (monocyte) cell line culture were more correlated with in vivo results than the results of L929 (fibroblast) cell line culture. Therefore, we could know that inflammatory cell lines are more effective in the evaluation for biocompatibility of medical devices.