원문정보
초록
영어
Over the last few years, gene delivery efficiency has been increased to study for the gene therapy. Adeno-associated viral (AAV) vectors are one of the most useful vectors for gene therapy. Therefore it has been influential in biomedical applications. However, AAV vectors have a major limitation for transduction efficiency in stem cells. Here, we report the Hybrid AAV Vector system to overcome the transduction limitations. AAV vectors are formation with cationic polymer PEI and 3,4-dihydroxy-L-phenylalanine (DOPA), and its delivery efficacy was studied in stem cells applications. Compacted hybrid AAV Vector has both sides of advantages each viral and non-viral vector. The AAV vectors are considerably safe and efficient gene delivery vehicles, and PEI is one of the useful cationic polymers that are effective in gene delivery because it displays not only an effective gene carrier but also a buffering capacity which provides a chance to escape from endosome. In addition, DOPA is the major components of mussel’ adhesive proteins, which forms strong covalent and non-covalent interactions with substrates. Hybrid vectors can be used as a powerful tool for gene targeting in neural stem cells.