원문정보
초록
영어
Microfracture of cartilage induces migration of bone marrow-derived mesenchymal stem cells (BMMSCs). However, this treatment often results in fibrocartilage regeneration. Bone morphogenetic protein (BMP)-2 induce the differentiation of BMMSCs into chondrocytes, which can be used for hyaline cartilage regeneration. We tested the hypothesis that long-term delivery of BMP-2 to cartilage defects subjected to microfracture results in regeneration of high quality hyaline-like cartilage. Heparin-conjugated fibrin (HCF) and normal fibrin were used as carriers for the long and short-term delivery of BMP-2, respectively. Rabbit articular cartilage defects were treated with microfracture combined with one of the following: no treatment, fibrin, short-term delivery of BMP-2, HCF or long-term delivery of BMP-2. Eight weeks after treatment, histological analysis and biochemical assay revealed that the long-term delivery of BMP-2 group had the highest GAG content and expression level of collagen type II. Taken together, the long-term delivery of BMP-2 to cartilage defects subjected to microfracture resulted in regeneration of hyaline-like cartilage.