earticle

영어

YIGSR (Tyr-Ile-Gly-Ser-Arg) and IKVAV (Ile-Lys-Val-Ala-Val), which are located in the β1 and α1 chain of laminin, have been demonstrated to promote cell adhesion and growth.[1] A thermoplastic polycarbonate urethane (TPU) is attractive in biomedical applications due to its outstanding physical properties, biostability, and biocompatibility.[2] In this study, we evaluated the effect of the YIGSR/IKVAV surface-conjugated two different morphogic TPUs for their specificity and growth promotability using nerve cell. Two morphologies of TPU substrates, film (MTPU) and aligned fiber (STPU), were prepared by solvent casting and electrospinning methods, respectively. To conjugate the peptides on the surface of TPUs, the substrates were activated by oxygen plasma treatment, and then poly(ethylene glycol) bisamine (PEG-BA) was grafted on the surface as a linker between the TPUs and the peptides. The resulting TPU surfaces were characterized by ATR-FTIR spectra, water contact angle measurement, and XPS. Protein adsorption experiment was carried out to evaluate the protein-resistance of the surfaces. The rate of protein adsorption was significantly reduced after modification. In addition, substrates were shown that have different influence on the nerve cell. The result was may occurred from different chain derivation. These laminin conjugated TPUs are expected that can use for dissimilar aimed neural engineering by means of distinctive interaction. Acknowledgement: This research was supported by the Pioneer Research Center Program through the National Research Foundation of Korea funded by the Ministry of Education, Science and Technology (2010-0019346).