원문정보
초록
영어
The combination of viral gene delivery and key features of biomaterial scaffolds that control viral delivery in a controlled manner greatly enable tissue engineering applications. In this study, adeno-associated virus (AAV), which have safety and efficient gene delivery capability in human gene therapy as therapeutic vectors, was encapsulated by electrospinning blended mixtures of elastin-like polypeptides (ELPs) and poly (e-caprolactone) (PCL) and was employed to transduce fibroblasts adhered on the scaffolds. Combinatorial interactions between ELP and PCL chains with varying ratios significantly altered wettability, elastic modulus and strain of the ELP/PCL composites. Then this system was useful tools to mediate controlled release of AAV vectors and efficient cellular transduction on the fibrous scaffolds. The capacity of ELP/PCL composites to modulate the controlled release of AAV-mediated gene delivery for subsequent high-efficiency cellular transduction will provide tremendous opportunities for a variety of tissue engineering applications.