earticle

영어

Mesenchymal stem cells are multipotent cells capable of differentiating into various mesenchymal tissues, such as bone, cartilage, fat, tendon and muscle. In order to explore their usages in medical applications, the ex vivo expansion of MSCs to sufficient cell numbers is necessary. The expansion of MSCs strongly depends on the culture conditions like pH, temperature, oxygen, basal medium, serum and other supplements. In this study, a newly-formulated in-house medium introduced for effective culture of mesenchymal stem cells and culture environments such as pH, oxygen, carbon dioxide in gas phase have been investigated. The new in-house medium showed much better performances in cell expansion as well as differentiation potential of cultured cells when compared with commercial media including DMEM, KSFM, MSCGM and Mesencult. Carbon dioxide content in gas phase needs to be carefully considered particularly when the widely used DMEM is introduced as a basal medium, since there is strong possibility of increased pH value even in common carbon dioxide condition of 5%. Oxygen content in gas phase also has shown the influence in the culture of MSC.