earticle

영어

2,3-butanediol (2,3-BD) is a very useful chemical, which is used as a solvent, a liquid fuel, and a precursor of many synthetic polymers. A 2,3-BD-producing bacterium, which was identified as Raoultella sp. B6, was isolated from an oil-contaminated soil sample. The isolate could produce 2,3-BD using various carbon sources. The effects of initial glucose concentration, initial pH, and temperature on 2,3-butanediol production were investigated using flask test. These parameters influenced the productivity and yield of 2,3-BD, and the production of the other fermentation products such as succinate, lactate, acetate, formic acid, acetic acid, and ethanol. The final 2,3-BD concentration was proportional to the initial glucose concentration. However, the productivity is inversely related to the initial glucose concentration. The growth rate and 2,3-BD production was the highest at neutral pH. The bacterium produced 2,3-BD at the start of the cell growth at 30 ℃, whereas it produced 2,3-BD in the middle of exponential phase at 25℃. Nevertheless, the highest concentration of 2,3-BD was the same in both cases. Based on these results, fed-batch culture was performed with or without aeration. This experiment showed that aeration has a positive effect on the cell growth and 2,3-BD production.