earticle

영어

To investigate the efficacy of Fe biosorption, bacterial communities in coal mine drainage were evaluated by constructing 16S rRNA gene libraries. The acid and organic solvent were used to increse adsorption ability on the surface of the cell membrane. acid (sulfuric acid, hydrochloric acid, and nitric acid) were used to activate functional groups of the outer cell membrane of Klebsiella oxytoca and to permeabilized cell and organic solvents (chloroform, toluene, acetone, and ethanol) activate the functional group of outer membrane and inner membrane of Klebsiella oxytoca. Treatment with 0.1% sulfuric acid was the most effective acid treatment, supported by results from SEM and FT-IR. Treatment with organic solvents resulted in lower permeability barriers due to falling out lipids of the cell membrane. Therefore, permeabilized cells showed higher absorption of iron with low cell viability. The residual amounts of phospholipids of the cell membrane were measured to investigate the mechanism of the improved permeability