earticle

영어

This study was carried out to improve the efficiency of conventional biopanning protocol based on batch adsorption. Chromatographic biopanning protocol using IDA monolithic column was used in search of copper ion binding peptide from a phage-displayed peptide library. Although a number of metal binding peptide sequences have been known, biopanning protocol using chromatographic matrix has hardly been reported. Chromatographic biopanning can be very effective way to find the strongest binders to target components fast and conveniently. After several rounds of positive and negative screening step, phage particles binding to copper were isolated