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By using green fluorescence protein as a selective marker of the successful transformed cells, we tried increasing the algae oil content by using glycerol-3-phosphate dehydrogenase gene (GPD1) from baker yeast with PIRES2-EGFP vector and Eppendorf Multiporator Electroporation System. We used mixed algae culture Scenedesmus spp. and Ankistrodesmus spp. in Beijernik medium. We attempted four different electro pulses 1.5, 1.8, 2, and 2.3 KV and results were analyzed by using fluorescence microscope with Nile Red and flow cytometry. The most successfully transformed species was Scenedesmus spp. at pulse rate of 2 KV. By using three filters of the flow cytometry detector FL1 vs. FL2 and FL3 vs. FL2, we found oil content in the transformed strain was significantly increased than wild type strain. However, after few generations, transformed cells with green fluorescence phenotype were very low (~20:30 cell per ml). Since we didn't add any type of antibiotics in the media to make selection and stabilization of the plasmid vector inside the algae cells, this may be the reason that we tested before the sensitivity of Scenedesmus spp. and Ankistrodesmus spp. mixed culture to kanamycin and neomycin antibiotics. We recorded wild type strains, used, can grow well on kanamycin and neomycin.