earticle

영어

K. pneumoniae wild type is capable of producing 2,3-butanediol from glucose by fermentation, but pH, oxygen rate, intracellular NAD+-NADH balance, and etc are the main obstacles. In order to obtain an microorganism with abundant 2,3-BDO yield, the wild type K. pneumonia were screened [1], [2]. Also 200ml flask fermentation with 36 g/L glucose feed at 37℃, 250 rpm was performed for wild type strain of the selected K. pneumoniae. As a result the selected K. pneumonia strain showed an promising 2,3-BDO productivity. In addition, in order to achieve the optimal medium condition for 2,3-BDO production, 200ml flask fermentation such as; temperature, glucose concentration, and etc, were conducted.