earticle

영어

Mircoalgae require proper conditions for the cell growth. Since microalgae are photosynthetic, proper light source, carbon dioxide concentration, water, and inorganic salts are required for optimal growth conditions. In this study, various light intensities and nitrate concentrations were tested with conditions of 20℃, 24:0 L:D cycle and 2.5 L/min aeration rate in 20 L batch culture. It took 5 days to reach the stationary phase for the culture of T. suecica with the light intensities of 108.9 and 133.1 μmol/m2/s with biomass of 0.89 and 0.88 g dcw/L, respectively. And, biomass productions of 1.07 and 1.00 g dcw/L were obtained with the nitrate concentrations of 18.55 and 24.74 mg/L, respectively. Nitrate was consumed by T. suecica of all tested groups within 5 days after the inoculation. Two-stage culture process through nitrate depletion led to increase oil contents from 7.6 % to 17.3 % (w/w) and contents of C16∼�18 fatty acids from 540.2 mg/g oil to 720.5 mg/g oil at 4th day after nitrate depletion. The biomass production and oil content of T. suecica was enhanced by the optimization of growth conditions and 2-stage culture process.