원문정보
초록
영어
Ethanol production was performed in simultaneous saccharification and fermentation (SSF) processes from a seaweed, Undaria pinnatifida (Sea mustard, Miyuk). To obtain a high ethanol yield, pretreatment conditions were optimized and marine bacteria treatment was performed. Pretreatment by thermal acid hydrolysis was carried out at different sulfuric acid concentration of 40.0-187.6mM H2SO4, treatment time of 15-60 min and solid contents of seaweed powder with 10-20% (w/v). Optimal pretreatment conditions were determined as 75mM H2SO4 and 13% slurry at 121℃ for 60 min. A maximum reducing sugar of 28.65 g/L and viscosity of 34.55 cP were obtained. The pretreated seaweed was further saccharified by the addition of 0.98 g dcw/L isolated marine bacteria dispersion after neutralization by 5N NaOH. The mixture were incubated at 30℃ with 200 rpm for 4 days. Reducing sugar of 41.73 g/L and viscosity of 32.18 cP were obtained. SSF was carried out to produce ethanol. The ethanol concentration of 3.26 g/L and viscosity of 22.66 cP were obtained by adding 0.98 g dcw/L isolated marine bacteria and 2.16 g dcw/L yeast, Candida tropicalis KCTC 7212, in SSF processes.