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Ethanol from seaweed, Saccharina japonica (Sea tangle, Dasima) was produced by the optimization of hydrolysis of macroalgae polysaccharide for the saccharification and simultaneous saccharification and fermentation (SSF) method. The seaweed was dried by hot-air and ground by hammer mill to 200 mesh s prior to the pretreatment. Saccharification was carried out by thermal acid hydrolysis using H2SO4 andIZE industrial enzyme, Termamyl 120 L. To increase the yield of saccharification, the isolation of marine bacteria was carried out. The optimal saccharification was carried out with thermal acid hydrolysis of 10% (w/v) seaweed slurry by 40mM H2SO4 and then the treatment of 1 g dcw/L isolated Bacillus sp. JS-1. The reducing sugar concentration and viscosity under the optimal saccharification procedure were 45.6±5.0 g/L and 24.9 cp, respectively. The yield of saccharification with the optimal condition from Saccharina japonica was 69.1%. SSF was carried out for the ethanol production. The highest ethanol concentration of 7.7 g/L with 33% of theoretical yield was obtained by SSF with 0.39 g dcw/L Bacillus sp. JS-1 and 0.45 g dcw/L yeast, Pichia angophorae KCTC 17574.