원문정보
초록
영어
The optimal agitation speed and aeration rate for mass production of carboxymethylcellulase (CMCase) of B. amyloliquifaciens DL-3 by E. coli JM109/DL-3 were established in a 7 L bioreactor using the response surface method. The analysis of variance (ANOVA) of results from central composite design (CCD) indicated that highly significant factor ("probe>F" less than 0.0001) for cell growth and production of CMCase was the aeration rate. The optimal agitation speed and aeration rate for cell growth were 498rpm and 1.4 vvm, whereas those for production of CMCase were 395 rpm and 1.1 vvm. The optimal inner pressure in a 100L bioreactor for cell growth was 0.8 kgf/cm2, whereas that for production of CMCase was 0.6 kgf/cm2. The maximal production CMCase by a recombinant E. coli JM109/DL-3 under optimized conditions in this study was 871.0 U/mL, which was2.4 times higher than that by B. amyloliquifaciens DL-3.