원문정보
초록
영어
Agar, which is accumulated in the cell walls of Red algae, such as Gelidium and Gracilaria is composed of agarose and agaropectin. In addition, agarose is composed of D-galactose and 3,6-anhydro-Lgalactopyranose with repeated structure. Agarase is agarolytic enzyme. This catalyzes the hydrolysis of agar and decomposes into agarooligosaccharides and neoagarooligosaccharides. The former is produced by α-agarase or acid catalysis. On the other hand, The latter is produced by β-agarase catalysis. In the previous study, agarase producing bacteria from the guts of spiny turban shells is isolated. For industrial application, the development of efficient culture condition and media composition is essential. This study sets a goal of both competitively mass production and the highest enzyme activity. Through one factor at a time(OFAT) method, the optimal temperature, pH, carbon source and the nitrogen source were determined. And then media composition was optimized by design of experiment(DOE). These results improved agarase production from Pseudoalteromonas sp. JYBCL 1.