원문정보
초록
영어
A marine bacterium possessing a novel activity of alginate degradation was isolated from a brown seaweed soup liquefied by salted and fermented anchovy. The isolated strain was designated as Brevundimonas sp. MJ-3 based on the analysis of biochemical characteristics and cellular fatty acid composition. The alginate lyase gene was cloned from genomic DNA library and expressed in Escherichia coli. The optimal pH and temperature for the recombinant alginate lyase were 7.0 and 40oC, respectively. When the multiple sequence and conserved domain were analyzed, interestingly, the cloned gene product was predicted to consist of alginate lyase L-like protein domain and Heparinase-like protein domain. The final degradation product of alginate was analyzed by ESI-MS and proved to be unsaturated monosaccharides. Based on the results, the cloned gene possessed an exotype alginate lyase activity that can degrade the alginate into monosaccharides. Acknowledgement : This work was supported by New &Renewable Energy R&D program (20093020090020) under the Korea Ministry of Knowledge Economy (MKE) and Korea Industrial Technology Foundation (KOTEF) through the Human Resource Training Project for Strategic Technology.