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영어

This study was to investigate the improvement of biological activities of Saposhnikovia divaricata through ultrasonification extraction process. Saposhnikovia divaricata was extracted at 60°C and 130 KHz for 30 min. The extraction yield was 26.1% (v/v) which was higher than that from convenational extraction using water at 100°C for 12 hours. All of extracts at a concentration of 1.0 mg/ml showed relatively low cytotoxicity on human normal kidney cell (HEK293) in range of 15∼ｦ25%. The inhibition ratios of several cancer cell lines such as human lung cancer cell A549, human gastric cancer cell AGS and human hepatocarcinoma cell Hep3B were measured using the sulforhodamine-B assay. The ultrasonification extracts of Saposhnikovia divaricata showed the highest cancer cell (A549, AGS, Hep3B) growth inhibition ratio as 77.3%, 79,1% and 75.9%, respectively. The immune B and T cell growth was improved by the ultrasonification extracts of Saposhnikovia divaricata up to 1.2×104 cells/mL and 1.0×104 cells/mL, respectively. The extract prepared also greatly increased the secretion of both IL-6 and TNF-α from ultrasonification process. This results can conclude that ultrasonification process effectively released active biomaterials which could important role in enhancing immune activity in the body.