원문정보
초록
영어
Guanosine-5’diphosphate (GDP)-L-fucose is an essential precursor for the synthesis of fucosyloligosaccharides, which are involved in various biological and pathological functions such as cell adhesion, inflammation, tumor metastasis and host immune response modulation. In this study, key genes for GDP-Lfucose production, encoding GDP-D-mannose-4,6-dehydratase (GMD) and GDP-4-keto-6-deoxy-D-mannose-3,5-epimerase-4-reductase (W caG) from Escherichia coli were introduced into C. glutamicum. GDP-L-fucose production was attempted by combinatorial overexpression of mannose-6-phosphate isomerase (manA), phosphomanno-mutase (manB) and GTP-mannose-1-phosphate guanylyl-transferase (manC) genes, which are also involved in GDP-D-mannose biosynthesis. Batch fermentations using the recombinant C. glutamicum systems showed that overexpression of gmd, wcaG, manB, manC was the most effective for GDP-L-fucose production and gave a 5.8-fold enhancement in specific GDP-L-fucose content compared with the control strain overexpressing GMD and WcaG only. A maximal GDP-L-fucose concentration of 86.2 mg/l was achieved in a batch fermentation of the recombinant C. glutamicum overexpressing GMD, WcaG, ManB and ManC.