earticle

영어

The goal of this study was to increase fatty acid production by developing recombinant E. coli strains which fatty acid synthesis (FAS) were improved. The acetyl-CoA carboxylase (ACC) enzyme from accA, accB and accC genes catalyzes the addition of CO2 to acetyl-CoA to generate malonyl-CoA. The enzyme encoded by the fabD gene converts malonyl-CoA to malonyl-[acp], and the EC3.1.2.14 gene converts fatty acyl-ACP chains to long chain fatty acids. Therefore, over-expression of acc family genes and EC3.1.2.14 gene was expected to increase the productivity of fatty acids. All recombinant E. coli strains containing various gene combinations to enhance the enzymatic activities were developed using the pTrc99A vector. The in vitro metabolites, amount of total lipid and fatty acids produced were analyzed to observe the changes in metabolism by introducing five distinct genes. This result indicated that from recombinant E, coli which had EC3.1.2.14 gene, the amount of total lipid and fatty acids were produced much more than the wild type E. coli.