earticle

영어

Since Escherichia coli requires homeostasis of internal pH in the range of 7.4 to 7.9, the pH response is important for cellular growth and survival under conditions of fluctuating pH [1,2]. The response and adaptation for pH change in E. coli strains were studied by proteome analysis. Comparison of their proteomes by separating two-dimensional gel electrophoresis following LC-MS/MS allowed us to identify 30 protein spots showing significantly altered levels in pH-dependent manner. The low pH responsive proteins included inner membrane proteins, membrane-associated proteins, outer membrane proteins and flagellum filament protein. This study shows that the different pH might induce drastic changes in composition and structure of E. coli membrane. [This work was supported by the Korean Systems Biology Research Grant (20090065571) of Ministry of Education, Science and Technology (MEST) to S.Y. Lee through the National Research Foundation (NRF), and the Basic Science Research Program (2010-0008826) from the NRF to M.-J. Han]