earticle

영어

The four enzymatic reactions of the elongation cycle in fatty acid synthesis metabolism were catalyzed by β-ketoacyl-acyl carrier protein (ACP) synthase (KAS) (FabB, FabF and FabH), β-ketoacyl-ACP reductase (FabG), β-hydroxyacyl-ACP dehydrase (FabZ), and enoyl-ACP reductase (FabI). These enzymes play an essential role in the extension of fatty acid length. To investigate cellular long-chain fatty acids composition in E. coli K-12 MG1655 the overexpressed strains of the fabG::fabZ or fabG::fabZ::fabI or fabG::fabZ::fabI::fabH genes were constructed. The culture optimization was tried to observe enhanced production of long-chain fatty acids. The fatty acids content and chain length were analysed in the recombinant E. coli strains and wild-type by using GC/MS. The results indicated that developed strains produced more cellular total lipid and fatty acids, and altered long-chain fatty acids composition.