원문정보
초록
영어
The draft genome of a hexanoic acid-producing bacterium Megasphaera sp. BS-4 was obtained using GS-FLX titanium and 441,216,354 total base pairs data was generated from 1,213,411 reads. Both fragment library and paired-end library were used as sequencing templates and one-fouth plate was used to generate sequence reads for each library. Assembly was carried out using Newbler and the assembled genome was annotated using RAST. The assembled genome was consisted with 7 scaffolds and 82 contigs. The approximate size and coverage of the genome was 2.8 Mb and 155X respectively. The G+C content of the genome was 48.99%. The genome contained 2,713 ORFs, 5 rRNA genes and 53 tRNA genes. The longest contig was 384,514 bp. A total of 1966 genes were categorized based on COG. Among these genes, 240 genes were assigned to ‘mino acid transport and metabolism’(E). ‘arbohydrate transport and metabolism’(G) and ‘nergy production and conversion genes’(C) were 111 genes and 152 genes.