earticle

영어

On the basis of the hypothesis that high fat diets (HFD) can induce gender-different obesity development, we attempted to unravel the gender-difference at a molecular level. To this end, we performed proteomic analysis in plasma of rats fed a HFD by 2-DE combined with MALDI-TOF-MS, thereby analyzed differential expression patterns between male and female plasma proteins. When exposed the rats to HFD, the body weight gain and food intake of HFD-fed rats were higher than those of low fat diets (LFD) rats in both genders, where male rats gained approximately 41% more body weight than female rats. These rats were used for proteomic analysis and changed 31 proteins among 370 visualized spots were identified by PMF. Changed proteins in each group were classified into three groups which were composed of proteins with gender difference in LFD and/or HFD groups (category I), changed proteins by HFD in male and/or female groups (category II) and proteins with both gender difference in LFD and/or HFD groups as well as HFD effect in LFD and/or HFD groups (category III). As a result, 7 proteins of category I, 13 proteins of category II and 11 proteins of category III were found. These findings suggest that these proteins may contribute to the gender differences in obesity development directly or indirectly.