earticle

영어

We constructed Candida antarctica lipase B 1422 (CalB1422, a mutated CalB with increased activity and secretion efficiency)-displaying yeast Saccharomyces cerevisiae by fusion of a-agglutinin anchor with the 5’å region of CalB1422. Cell-surface display of the CalB1422 fusion protein was confirmed by both p-nitrophenyl palmitate assay and flow cytometry after staining the cells with antibody against the CalB1422. CalB1422 fusion protein was observed with higher fluorescence intensity on the surface of the yeast than an yeast non-displayed CalB1422. The CalB1422 was displayed functionally on the cell surface of S. cerevisiae using the anchor protein a-agglutinin. These results suggested that CalB1422-displaying cells could be useful as a whole cell biocatalyst for the production of lipase-catalyzed products, such as enantioselective chemicals and biodiesel.