earticle

영어

Heterologous protein production is becoming an important field in biotechnology as a source of many different kinds of proteins, including therapeutic proteins. Traditionally, these proteins were produced through the use of small model multi-cellular organisms, but these systems are giving away to microorganisms, such as the yeast P. pastoris, due to their rapid doubling time and lower cost in growth media. Additionally, the ease in which the proteins can be harvested makes P. pastoris and attractive host. We have constructed the genome-scale metabolic model of the methylotrophic yeast P. pastoris and incorporated additional biochemical reactions to represent the production of two proteins, human serum albumin (HSA) and human superoxide dismutase (hSOD). Using this metabolic model, we analyzed the yeast’ capacity for producing proteins and performed simulations to suggest metabolic engineering targets to improve production. [This work was supported by the Korean Systems Biology Research Project (20100002164) of the Ministry of Education, Science and Technology (MEST) through the National Research Foundation of Korea. Further support by the World Class University Program (R32-2008-000-10142-0) through the National Research Foundation of Korea funded by the MEST is appreciated.]