원문정보
초록
영어
Isoprene (2-methyl-1,3-butadiene) is a volatile C5 terpenoid released mainly from leaves of many deciduous trees and some bacteria. Isoprene is present as a colorless liquid under standard conditions. Also, it is the monomer of natural rubber and a precursor to an immense variety of naturally occurring terpenoids. Isoprene is an important chemical feedstock used in the synthetic rubber industry. About 800,000 tons per year of cis-polyisoprene are produced from the polymerization of isoprene, and most of this polyisoprene is used in the tire and rubber industry. Isoprene is made from dimethylallyl diphosphate (DMAPP) by isoprene synthase via methyl erythritol 1-phosphate pathway (MEP) in plants. In this study, we constructed plasmid containing isoprene synthase (ispS) from three kinds of plant, Populus alba, Populus trichocarpa, and Pueraria Montana (kudzu) and introduced into E. coli to produce isoprene. By GC analysis, we confirmed isoprene production in E. coli. To produce more isoprene, we introduced pSNA plasmid containing whole mevalonate pathway for DMAPP synthesis and codon optimized ispS gene into E. coli. This work was supported by a grant (NRF-2010-C1AAA001-0029084) from the National Research Foundation (MEST), the KRIBB Research Initiative Program, and BK21 program of Korea.