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영어

Although 1.42 million single-nucleotide polymorphisms (SNPs) were identified in human genome, the relationships between diseases and SNPs have not been clarified. For this reason, high-throughput genotyping methods are needed for analyzing human genes and many researchers are searching for various and effective detection methods. In this study, we prepared repetitive polypeptides for SNP detection using capillary electrophoresis in free-solution. We demonstrate a method for multiplexed single-base extension (SBE) genotyping that takes advantage of the unique separation modalities made possible via end-labeled free-solution electrophoresis (ELFSE). A series of “rag-tags”was created using biological synthesis and used to achieve DNA separation by microchannel electrophoresis without a polymeric sieving matrix. This method is shown to be applicable for SNP genotyping and expected to search for the various genetic diseases..