earticle

영어

Saccharomyces cerevisiae is widely known and used as an industrial strain especially for the production of ethanol. Ethanol is the most important biofuel these days, while a lot of longer chain alcohols are being developed as commodity chemicals. 2,3-butanediol, as one of the longer chain alcohols, is a chemical with multiple practical applications. It is naturally produced by microorganisms such as Klebsiella pneumonia, Klebsiella oxytoca and Enterobacter aerogenes. (1) We attempted to engineer S. cerevisiae for 2,3-butanediol production. In an attempt to redirect carbon flux towards 2,3-butanediol production, we have devised two metabolic engineering strategies. For each strategy, we have constructed a variety of strains by applying loxP/Cre mediated PCR-based gene deletion method and also gene overexpression technique. In the first strategy, pyruvate decarboxylase genes (PDC1, PDC5, PDC6) were deleted. In the second strategy, ADH1 gene (encoding alcohol dehydrogenase) and ALD6 gene (encoding major cytosolic acetaldehyde dehydrogenase) are deleted from the wild type strain. Then, acetolactate synthase from Bacillus subtilis (AlsS) and/or butanediol dehydrogenase (BDH1) gene were overexpressed. We compare the production of 2,3-butanediol from both strategies.