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영어

There has recently been much research conducted on the high temperature activity and thermal stability of reverse transcriptases. Traditional M-MLV reverse transcriptase possesses low thermal stability, allowing for reverse transcription reactions to occur only at relatively low temperatures (42℃). This limitation prevents the efficient transcription of RNA molecules with complex secondary structures. To resolve this, BIoneer has utilized synthetic biotechnology and protein engineering to develop a thermostable reverse transcriptase with activity at high temperatures of 50 °C and above, and dubbed the enzyme RocketScript. Higher temperature reverse transcription reactions allow optimization of cDNA synthesis experiments with temperature. RocketScript is Bioneer’ exclusive M-MLV-based thermostable reverse transcriptase (RTase). Because the original M-MLV RTase has maximum activity at relatively low temperatures (42 °C), it caused several problems during reverse transcription of complex secondary structure RNA species. RocketScript has thermostable activity (42~70°C), allowing efficient cDNA synthesis from complex secondary structure RNA.