원문정보
초록
영어
An aerobic bacteria termed AN-C16-KBRB was isolated from bovine rumen and demonstrated cellulolytic activity on a NB agar plate containing azo-carboxymethyl cellulose. The novel celEdx16 gene encoding a cellulase was cloned, sequenced, and expressed in E. coli, and the enzyme was characterized. CelEdx16 showed approximately 50% identity with known cellulolytic enzymes of various microbes including C. thermocellum, C. cellulovorans, P. rhizinflatus, N. patriciarum, R. albus, and O. joyonii. CelEdx16 was active on MeUmbG2, a fluorogenic glycoside substrate of exo-type cellulases as well as CMC. The CelEdx16 cellulase is of considerable interest because the enzyme is bifunctional, and is stable both in the presence of metal ions and under strongly alkaline conditions. (This work was supported by a high throughput screening (HTS)-based Integrated Technology Development grant from the MEST, and a basic research grant from KRIBB.)